|
KMID : 0381120210430020161
|
|
Genes and Genomics
2021 Volume.43 No. 2 p.161 ~ p.171
|
|
|
The miR-145-5p/CD36 pathway mediates PCB2-induced apoptosis in MCF-7 cells
|
|
Yuan Yuan
Xue Caihua
Wu Qiang
Wang Mengjie
Liu Jiahua
Zhang Longfei
Xing Qianwen
Liang Jingyan
Wu Hua
Chen Zhi
|
|
|
Abstract
|
|
|
Background: Procyanidin B2 (PCB2) can increase the levels of anti-inflammatory and immune mediators.
Objectives: However, its molecular mechanism in human breast cancer remains unclear. This study aimed to investigate the antitumor effect of PCB2 on MCF-7 cells and to examine the underlying mechanism.
Methods: The flow cytometry and EdU incorporation assays were measured the PCB2-induced BMECs. The expression levels of inflammatory factors and immune response genes were upregulated in MCF-7 cells, high-throughput sequencing was used to detect differentially expressed genes in blank and PCB2-treated MCF-7 cells.
Results: The results showed that PCB2 induced the apoptosis of MCF-7 cells. CD36 profiles were affected in MCF-7 cells. Additionally, prediction software identified a miR-145-5p binding site in the CD36 sequence. Luciferase reporter assays and Western blot analysis were used to verify the regulatory relationships between the differentially expressed miRNA miR-145-5p and CD36. MiR-145-5p and its key target (CD36) constitute a potential miRNA-mRNA regulatory pair. Functional studies in MCF-7 cells revealed that CD36 promotes but miR-145-5p inhibits apoptosis.
Conclusion: Overall, these data suggest that miR-145-5p inhibits the enhancing effect of PCB2 on CD36 expression by binding CD36 and subsequently regulating apoptosis, the immune response and anti-inflammatory pathways. These results provide theoretical and experimental support for the treatment of breast cancer.
|
|
|
KEYWORD
|
|
|
Procyanidin B2, MCF-7 cells, Apoptosis, Molecular mechanism
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|